Performance of different phenotypic tests in detecting mecA mediated methicillin resistance in Coagulase negative staphylococci (CoNS)
نویسندگان
چکیده
Accurate detection of methicillin resistance (MR) in coagulase negative staphylococci (CoNS) is of utmost importance as infections due to CoNS are on the rise. Various phenotypic methods have been described for detecting mecA mediated MR with varying performance characteristics. The present study was designed to evaluate the performance of oxacillin and cefoxitin disc diffusion / MIC test and oxacillin screen agar against NAAT for detecting mecA mediated MR in 150 strains of CoNS. An inexpensive test to determine methicillin resistance reliably is of great value as NAAT are not easily available in all settings. In this study, cefoxitin disc diffusion accurately identified MRCoNS and can be used as a reliable test to determine the same.
منابع مشابه
A study of coagulase-negative staphylococci (CoNS) isolated from bovine mastitis for the presence of penicillin and methicillin resistance-encoding genes in the north west of Iran
Coagulase-negative staphylococci (CoNS) are often associated with bovine mastitis and may be resistant to antimicrobial therapy. The aim of the current study was to investigate the presence of blaZ (responsible for penicillin resistance) and mecA (responsible for methicillin resistance) genes among 108 CoNS belonging to 9 different species isolated from bovine mastitis in seven dairy herds (H1-...
متن کاملDifferentiationo Methicillin-Resistant and Methicillin-Susceptible Staphylococcus Aureus and Coagulase-Negative Staphylococci using Cefoxitin
Detection of the mecA gene or PBP2a is considered the gold standard for detecting mecA-mediated methicillin resistance in staphylococci. Among available phenotypic methods, the Clinical and Laboratory Standards Institute (CLSI) has introduced the cefoxitin disk diffusion (DD) test for predicting the presence of mecA in S. aureus and CoNS, which is preferred over the oxacillin DD test. Automated...
متن کاملIdentification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis.
OBJECTIVES This study focused on the correlation between geno- and phenotypic tests in the correct assessment of mecA-mediated methicillin resistance among coagulase-negative staphylococci (CoNS) and the further characterization of mecA-positive isolates. METHODS A total of 121 CoNS from cases of bovine mastitis were investigated for oxacillin susceptibility by disc diffusion and broth microd...
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The EVIGENE MRSA Detection Kit was evaluated on coagulase-negative staphylococci (CoNS) from agar plates and on staphylococci directly from positive spiked blood cultures. For the CoNS study, a total of 242 isolates were tested, and of these 237 gave valid test results. For the 237 valid tests, all gave correct mecA classification. For the blood culture procedure, a collection of 51 mecA-positi...
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The accurate performance of the Vitek 2 GP67 card for detecting methicillin-resistant coagulase-negative staphylococci (CoNS) is not known. We prospectively determined the ability of the Vitek 2 GP67 card to accurately detect methicillin-resistant CoNS, with mecA PCR results used as the gold standard for a 4-month period in 2012. Included in the study were 240 consecutively collected nonduplica...
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